|
KMID : 0358319960370060607
|
|
Korean Journal of Urology
1996 Volume.37 No. 6 p.607 ~ p.618
|
|
|
The Role of nm23-H1 Gene in Urologic Cancer(2): Expression and Multation of nm23-H1 Gene in Urothelial Cancer
|
|
|
|
|
Abstract
|
|
|
Background and Purpose:
@EN We had previously demonstrated by immunohistochedmica study 9IHS0 that nm23-H1 gene may play an important role in disease prognosis as well as its participation in metastasis of urothelial cancer. The purpose of present study was 1) to
reexamine the
role of nm-23H1 gene in urothelial canceress at molecular level, 2) to identify the molecular mechanism of decreased immunoreactivity for nm230H1 protein in metastatic urothelial cancers, and 3) to identify whether HIS is reliable in studying the
expression of nm23-H1.
@ES Materials and Methods:
@EN We studied expression level and mutation profiles of nm23-H1 gene in 25 fresh surgsical specimens of urothelial cancer by reverse transcription polymerase chain reaction(RT-PCDR)-Southern blotting analysis, and PCR of genomic DNA, followed by
singsle strand conformation polymorphism and sequencing analysis. The rresults of RT-PCR-Southem blotting were comparatively analyzed with those of HIS.
@ES Results:
@EN mRNA transcript levels of nm23-H1gene were also significnatly decreased in metastatic tumor tissues as compared with primary tumor tissues. Point mutation of nm23-H1 gene was detected in only 1 of 13 urothelial cancer tissues with metastasis,
whereas, mutation was observed in none of those without metastasis. The results of HIS corrdesponded with those of RT-PCR-Saouthern blotting analysis in 23 of 25 spe3cimens.
@ES Conclusions:
@EN The nm23-H1 gene may play an important role in metastasis of urotheilial cnacer. Decreased transeription at mPNA level may be a major molecular mechanism of loss of immunoreactivity for nm23-H1 protein in orothelial cnacer. HIS used in the
present
study may be a clinically useful dmethod to study the expression of nm23-H1 and to predict dmetastasis potential and prognosis of durothelial cancers.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
  
|
|
|
Listed journal information
|
|
  
|